Effect of Laser Irradiation on Diabetic Wounded Fibroblast Cells in Vitro
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چکیده
Diabetes is known to be associated with impaired wound healing, and loss of collagen related to diabetes may be due to decreased levels of synthesis or enhanced metabolism of newly synthesized collagen or both. This study aims to determine if laser irradiation stimulates cellular proliferation, nitric oxide (NO) and collagen synthesis in diabetic fibroblast cells. Induced diabetic wounded human fibroblast cells were irradiated at 830 nm with 5 J/cm2. Post-laser irradiation there was a significant increase in proliferation at 24 and 48 h and reactive oxygen species and NO generation at 15 min. There was no change in collagen type I. Laser irradiation at 830 nm with 5 J/cm significantly stimulates cellular proliferation and NO synthesis in diabetic wounded fibroblast cells.
منابع مشابه
Irradiation with a 632.8 nm helium-neon laser with 5 J/cm2 stimulates proliferation and expression of interleukin-6 in diabetic wounded fibroblast cells.
BACKGROUND The use of lasers has been shown to stimulate wound healing in vivo and in vitro. There is an increase in wound closure, cell viability, proliferation, and cytokine expression. If laser parameters can be optimized and standardized, and the underlying mechanisms better understood, this phototherapy can become an alternative safe treatment to slow-to-heal wounds, such as in patients wi...
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BACKGROUND AND OBJECTIVE Wound healing in diabetic patients remains a chief problem in the clinical setting and there is a strong need for the development of new, safe, reliable therapies. This study aimed to establish the effect of irradiating diabetic wounded fibroblast cells (WS1) in vitro on pro-inflammatory cytokines and the production of nitric oxide (NO). MATERIALS AND METHODS Normal, ...
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Objective(s): Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collage...
متن کاملThe Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study
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تاریخ انتشار 2008